Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR- SSCP technique
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چکیده
منابع مشابه
Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR-SSCP technique.
For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we designed two PNA molecules comprising aa 246-250 of exon 7 and aa 270-275 of exon 8, respectively, to...
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higher MetHb values in the presence of HbF. For MetHb concentrations of 15% or less, i.e., values most likely to be encountered clinically with NO therapy, the Corning 270, in agreement with the study carried out previously (5), was unaffected by the presence of HbF in the umbilical cord sample (P 5 0.7380). By contrast, the Chiron 800 showed a constant error to significantly higher MetHb (P 5 ...
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Efficient detection of somatic mutations is important for the development of clinical molecular diagnostic assays. However, the detection of somatic mutations in tissue is confounded by dilution of the tumor cell population by normal cells. Laser microdissection allows enrichment for tumor-associated genetic alterations to take place at the level of cell selection, eliminating the need to enric...
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T e c h n i c a l progress in the determination of DNA sequence has resulted in an enormous amount of DNA sequence data, with technical innovations such as the polymerase chain reaction (PCR) accelerating the rate of increase of this data. This combination of data and techniques makes it possible now to exploit sequence differences between individuals. Such studies not only can answer biologica...
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T e c h n i c a l progress in the determination of DNA sequence has resulted in an enormous amount of DNA sequence data, with technical innovations such as the polymerase chain reaction (PCR) accelerating the rate of increase of this data. This combination of data and techniques makes it possible now to exploit sequence differences between individuals. Such studies not only can answer biologica...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 1362-4962
DOI: 10.1093/nar/26.5.1356